Clonesmarter
WebThe Smart Way to Discover Cannabis & Hemp Genetics. Search for Clones, Teens, Mothers, Seeds, and Tissue Cultures WebJul 2, 2024 · After PCR amplification, the DNA fragments were cloned into a pCloneEZ-blunt plasmid vector (CloneSmarter, USA), and then transformed into E. coli DH5α competent cells (Weidi, China). The genome of GD-61 was assembled by SeqMan 7.1.0 based on the sequencing raw data.
Clonesmarter
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WebUse CloneSmart to see live inventories of cannabis clones available for purchase at your local retail dispensaries. Also find available hemp/ CBD clones. WebFeb 3, 2024 · PCR was performed to amplify the region flanked by the target site, and the 736 bp PCR products were subcloned into pCloneEZ vector (CloneSmarter), transformed into competent cells, and at least 20 clones were picked separately for Sanger sequencing. The sequences were aligned with DNAMAN multiple sequence alignment tools.
WebWithout competent cell[/shortdesc] [els_overview]CloneSmarter™ Zero Background Blunt Topoisomerase Cloning Kit is designed for fast cloning of blunt ended DNA fragments up … WebI planed to use gibson assembly and the enzyme I used was from clonesmarter (Seamless Assembly cloning kit) and Yeasen (Universal One Step Cloning Kit). I seperated my 9kb fragment into three 3k...
WebSupplementaryFigures FigureS1.OptimizationofSEAreaction. A.Optimizationoftheconcentrationofprimer.Thereactionmixturecontained1.0×10-9MHCV DNA,2.0×10-7M,5.0×10 ... WebRead reviews, compare customer ratings, see screenshots, and learn more about Clone Master. Download Clone Master and enjoy it on your iPhone, iPad, and iPod touch.
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