Command samtools not found
WebSamtools does not retrieve the entire alignment file unless it is asked to do so. If an index is needed, samtools looks for the index suffix appended to the filename, and if that isn't found it tries again without the file- name suffix (for example in.bam.bai followed by in.bai). WebFeb 12, 2024 · I am trying to run the following script on slurm to extract information from .sam files. While everything is perfect on my local machine with the samtools version …
Command samtools not found
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WebMar 15, 2024 · Hmm, I'm not sure sorry. The main branch of coverm now no longer uses remove_minimap2_duplicated_headers but requires minimap2 2.20 - you could try that. However my suspicion is still that it is running out of RAM - does it give a different result when you request say double the RAM in your (PBS?) queue submission? WebAug 14, 2015 · I had the same issue and found that there was no perl at #!/usr/bin/perl. neither perl -v or /usr/bin/perl -v were working. I did a find / -name for perl then added a symbolic link from /usr/bin to the real location. perl -v -bash: perl: command not found /usr/bin/perl -v -bash: /usr/bin/perl: No such file or directory
WebAug 28, 2024 · Furthermore, {SAMTOOLS} here is being accessed as a Snakemake wildcard, yet you do not use it as a wildcard in the rule's output's. Assuming that {wildcards.chr} works, and that the {SAMTOOLS} call was the only wildcard not being found (not just the first unknown wildcard), I think you should try either of two things. No … WebSep 21, 2024 · This error message indicates (admittedly somewhat obscurely) that the reads are not sorted by coordinate — in particular, there are mapped reads appearing after the …
WebWhen this option is in use, the input file may not be sorted. -T, --targets FILE Similar to -R but the entire input will be read sequentially and regions not listed in FILE will be skipped. -D Do not download the index file before opening it. Valid for remote files only. --cache INT Set the BGZF block cache size to INT megabytes. [10] WebAug 21, 2024 · Hi Brian, Sorry if you get this twice. Initially I had some problem in transferring abundance estimates to matrix. After searching for the same problem, I found your answer which fixed my problem by downloading a newer version of abundance_estimate_to_matrix.pl file with a support_script folder.
WebSep 21, 2024 · This error message indicates (admittedly somewhat obscurely) that the reads are not sorted by coordinate — in particular, there are mapped reads appearing after the unplaced unmapped reads (those with RNAME *) that should be at the very end of the file. This is because samtools sort -n has been used to sort the reads by name instead.
WebAug 6, 2024 · Short description of the problem After updating conda and Anvio to the latest version, samtools had an issue with not finding a shared library libcrypto.so.1.0.0 anvi'o version anvi-self-test --ver... citations for a research papercitationsharesWebGitHub - samtools/samtools: Tools (written in C using htslib) for manipulating next-generation sequencing data samtools / samtools Public 12 branches 61 tags Go to file jkbonfield Fix a sort -M bug when merging sub-blocks. 1eee54d yesterday 2,554 commits .ci_helpers Fix ci clone with no referenced HTSlib PR 2 months ago .github/ … diana state board of educationWebNov 1, 2024 · samtools image not found. Abort trap 6 · Issue #974 · samtools/samtools · GitHub I am using samtools 1.7-1 under anaconda on Mac OS High Sierra. I have not changed anything recently but suddenly samtools is not executing. It shows the below error on command line when samtools is executed in terminal. Last login: Thu ... citation shakespeare sur la vieWebSep 24, 2011 · First of all, I'm trying to get used to makefiles but yet I#m new with this. The following file is supposed to, first, compile all ./src/*.cpp files to ./src/*.o (where the filename survives) and diana stewart-brownWebI installed BWA and samtools from tool_shed, but I got the following error: Traceback (most recent call last): File "/local/galaxy-dist/lib/galaxy/jobs/runners/local.py", line 129, in … diana story wife of ralph storyWebMar 6, 2024 · [E::idx_find_and_load] Could not retrieve index file for 'calls_to_draft.bam' Why that would be the case is not clear to me. I should say medaka==0.11.5 is fairly old at this point, If you can upgrade to the latest 1.2.3 (on bioconda) or 1.2.4 (on pypi) we will be able to prvide further assistance. citations for laws