WebSep 1, 2024 · Electroporated and cryopreserved DCs were thawed, allowed to recover for at least 1 h at 37°C and 5% CO 2 in warm RPMI-GlutaMAX medium (Invitrogen by Life Technologies) supplemented with 10% huAB serum (Invitrogen by Life Technologies) and 100 U/mL P/S (Gibco by Life Technologies) and used as stimulators. As responders, … WebMay 29, 2016 · OptiMEM reduced serum medium without phenol red. 2. 4-mm electroporation cuvettes. 3. Gene Pulser XCell electroporator (Bio-Rad). 4. DC culture medium: RPMI-1640 medium containing 1 % huAB serum and 100 U/mL penicillin, and 20 mM l-glutamine. 5. 15 mL tubes. 6. Phosphate buffered saline (PBS). 7. In vitro …
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WebMar 29, 2004 · Medium containing HuAB serum enhanced the ability to measure tumor-reactive T cells. Pre- (white columns) and postvaccine (grey columns) PBMC (1 × 10 5 cells/well) were co-cultured with sCD40Lt activated autologous tumor cells (2 × 10 5 cells/well) for 48 h. The optimal media for the tumor IFN-γ ELISPOT assay was … WebNov 14, 2001 · DPBS/well and were blocked with 200 l/well RPMI 1640, 10% HuAB serum, 25 mM HEPES, 100 U/ml penicillin, 100 g/ml streptomycin, and 2 mM glutamine for1hat37°Cin5%CO 2. Flt3L-mobilized PBMC cultured with or without CD40L and IFN- (1 105 cells/well) and 1 510 allogeneic oligo-polyclonal tumor-infiltrating lymphocytes (TILs; … elmington.edge2learn.com
Ex vivo expansion of human T cells for adoptive immunotherapy …
WebApr 10, 2024 · 10.1 Future Forecast of the Global Recombinant Human Serum Albumin Market from 2024-2030 Segment by Region 10.2 Global Recombinant Human Serum … WebMar 15, 2003 · PBL (3 × 10 7) were incubated with 2 × 10 7 anti-CD4 Dynabeads in 2 ml of PBS plus 2% HuAB serum at 4°C for 1 h. Dynabead-rosetted CD4 + T cells were magnetically separated, washed five times with cold PBS plus 2% HuAB serum, and subsequently recovered by incubation with 60 μl of CD4-Detachabead reagent (Dynal) in … WebJan 28, 2016 · The day before performing an ADCC assay, PBMCs were thawed and activated with 600 IU/ml recombinant human IL-2 (Prometheus Laboratories Inc., San Diego, CA, USA) in RPMI-1640/10% huAB serum ... elmington edge2learn