Pcass2
Splet01. sep. 2006 · The pCASS2 vector [11], a gift from Dr. X. W. Ding, Singapore National Institute of Molecular Agriculture Research, was cut by StuI and dephosphorylated at the 5 -terminus, and then the full-length cDNA was ligated into the vector at the StuI site. The … SpletA full-length cDNA clone of the RNA genome of carnation mottle carmovirus Shanghai isolate (CarMV-sh) was obtained by ligating four fragments of cDNA together, and constructed downstream from partially doubled cauliflower mosaic virus 35S RNA promoter, generating a transient expression plasmid pCaCarMV derived from a pUC …
Pcass2
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SpletVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene. This page is informational only - this vector is NOT available … SpletComplete genome sequence cloned in pCass2 vector. Unit: 364 ng/ul, 10ul in microtube Riboviria / Orthornavirae / Kitrinoviricota / Alsuviricetes / Tymovirales / Betaflexiviridae / Trivirinae / Trichovirus / Grapevine Pinot gris virus
SpletTRV2 encodes coat protein from the genomic and two nonstructural proteins from subgenomic RNAs (A). To develop the TRV-based VIGS vector, a cDNA clone of TRV1 or TRV2 (Ppk20 strain) was placed between a duplicated CaMV 35S promoter (2X35S) and … Splet20. jul. 2005 · To facilitate construction of T-DNA-based plasmids, a general vector pCass4-Rz (Fig. 1 A), a variant of pCass2 (Shi et al., 1997), was constructed by amplifying a PCR product encompassing multiple cloning site consisting of five restriction sites and a 68-nt fragment encompassing the ribozyme (Rz) sequence of a satellite RNA of tobacco ring ...
Splet03. apr. 2000 · This fragment was ligated into similarly digested pCass2 to give pGFP. XbaI and NcoI restriction enzyme sites were introduced by PCR onto the 5′ and 3′ ends, respectively, of the CMV 2b open reading frame (ORF) (Q strain) as encoded by pSK2b (Ding et al., 1994) and the resultant fragment ligated into similarly digested pGFP to yield … SpletBioVector NTCC Inc. 订购电话TEL:400-800-2947 工作QQ/微信:1843439339 Email:[email protected]
SpletComplete genome sequence cloned in pCass2 vector. (PCR products, Bulk positive control, DNA template for recombinant virus or replicon, Diagnostic reagent, Derived product, Nucleic Acid, Cloned nucleic acid)
SpletpOK12 of the GVB infectious clone pCass2 FL (Sal-darelli et al., 2000) was modified by PCR introducing a stop codon in the CP gene, thus terminating the CP polypeptide after 50 aminoacids. The full-length clone was restored by ligation and 0.8 µg of purified DNA … girgit series downloadhttp://www.bioline.org.br/request?as03004 fun anthologySpletDownload scientific diagram The DNA regions of the NMV vectors cloned into pCass2 between the StuI and BamH1cloning sites. RdRp: RNA-dependent RNA polymerase; TGB: Triple gene block proteins; Ps ... fun anti bullying gamesSpletFull-length cDNA copies of the genomes of Grapevine virus A (GVA) and Grapevine virus B (GVB) under the control of bacteriophage T7 promoter have been synthesized, which were refractory to cloning in Escherichia coli. However, both transcribed cDNAs were … girg nuea facebookSplet01. jul. 2024 · PCass2 vector used for cloning genomic RNA of ArMV to produce viral infectious clones (full length clones of virus RNA1 and RNA2, as well as the sub viral RNA). b. PCR amplification of the viral satellite clone modified or not with GFP insertion into BamHI cloning site. Digestion profile with BamHI gives a fragment of about 700 bp … fun apis for projectsSpletcloned into pCass2 plant expression vector. The transcription of PRSV genome was driven by Cauliflower mosaic virus (CaMV) 35S promoter and terminated by CaMV terminator. Two full-length cDNA clones of PRSV-SMK5 isolate, which were designated as pCF17_7 and … fun anniversary vacation ideashttp://agscij.agr.ku.ac.th/phocadownload/2560-48-2/ASJ-48-2-208-220.pdf fun anniversary trip ideas